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ECACC catalog code: 87031301
Description: Rat hepatoma Reuber H35
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Hormone induced enzymatic studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Exhibits high arylhydrocarbon hydroxylase inducibility and is used to detect picogram levels of planar polychlorinated organic compounds in food extracts and other environmental samples.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1548, IZSBS: BS TCL 136
- Biochem Pharmacol 1973;22:2766 - PMID: 4357894
- In Vitro 1980;16:230
- Natl Cancer Inst Monogr 1964;13:229
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.