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SPECIES: human, Caucasian
ECACC catalog code: 95090714
Description: Human Caucasian colon adenocarcinoma
Viruses: See Properties
Depositor: N Solic, Dr D E Davies, Dr J E Collins, CRC Wessex Medical Oncology Unit, University of Southampton, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Epidermal growth factor (EGF) Applications: Colon tumour assays, influence of growth factors and adhesion molecules on colonic tumour cells
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:8 to 1:10 i.e. seeding at 5x10,000 to 1x100,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
GP2d has been established from the same adenocarcinoma as GP5d (95090715). The cells were derived from a local recurrence of Duke's grade B, poorly differentiated carcinoma of the colon from a 71 year old female at surgical resection. The patient received no preoperative chemo or radiotherapy and had a strong family history of colon cancer with two first degree relatives dying of the disease below the age of 55 years. Both clones possess genetic changes consistent with the pattern of tumour progression in colon cancer but are morphologically distinct. An interstitial deletion on chromosome 5 (region 14q to 22q) and an inverted duplication of bands 10q11 and 10q21 has been reported. Both cell lines contain a normal copy of the ki-ras gene and a copy with a transition in codon 12. GP2d show growth response to EGF, TGF alpha or insulin with an increase in cell numbers. Amphiregulin mRNA is abundant in GP2d but almost undetectable in GP5d.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Int J Cancer 1995;62:48 - PMID: 7601566
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Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.