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TISSUE/ORGAN: aorta, endothelium
DSMZ catalog code: ACC 109
Morphology: cells grow as adherent multilayer
Transformed by: Chemical Agent Back To Chemical Agents' WebPage
Description: bovine endothelial aorta established from a male bovine fetus at 24-week gestation; vascular endothelium transformed with benzo(a)pyrene in vitro; cell line described as tumorigenic the species human, mouse and rat were excluded with IEF of LDH, NP, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Prof. Presta, University of Brescia, Brescia, Italy
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% MEM (with Earle's salts) + 20% FBS + non-essential amino acids + MEM vitamins split confluent culture 1:2 to 1:3 every 3-6 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of about 0.8 x 105 cells/cm 2;
doubling time of about 30-60 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2.0 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypodiploid bovine karyotype with 15% polyploidy; 58(54- 60)<2n>; centric fusion and large submetacentric marker; corresponds to published karyotype) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * isoenzymes (the species human, mouse and rat were excluded by IEF using LDH, NP, PEP B) * reverse transcriptase (reverse transcriptase not detected)
- J Cell Physiol 1983;114:328 - PMID: 6833405
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.