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DSMZ catalog code: ACC 250
Morphology: epithelial-like adherent cells growing in monolayers
Description: human rhabdomyosarcoma established from the rhabdomyosarcoma of a 1-year-old girl in 1973; reported to form small malignant tumors in nude mice consistent with embryonic rhabdomyosarcoma confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: obtained from DKFZ Tumorbank, Heidelberg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85-90% McCoy's 5A + 10-15% FBS split confluent culture 1 x per week at up to 1:5 using trypsin/EDTA; seed out directly in a 80 cm 2 culture flask, cells will reach confluency within 5-8 days; cells may clump after trypsinization and may be difficult to disperse at 37C with 5% CO2 cell harvest of about 5-10 x 106 cells/80 cm 2;
doubling time of about 36 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human near diploid karyotype with tetraploid sideline (31%) and 3.5% polyploidy; 46(41-46)<2n>XX; no consistent chromosome abnormality detected) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin- desmin- endothel- GFAP- neurofilament- vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: HTB 82, ICLC: HTL99002
- J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.