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ECACC catalog code: 89072612
Description: Lizard Gekko lung
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Chromosome and virus studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 30C.
Karyotype: Modal No 37 and 38
From adult male Tokay Gekko (indigenous to Southeast Asia). Observed to undergo spontaneous chromosomal alterations from a normal diploid number of 38 to a bimodal distribution of chromosome number 38-40. Susceptible to a number of mammalian viruses at 36C.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 111
- Cytogenetics 1968;7:16-26 - PMID: 5659169
- Proc Soc Exp Biol Med 1970;133:1039-1047 - PMID: 4314147
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.