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SPECIES: rat, Wistar-Furth
TUMOR: pituitary tumor
ECACC catalog code: 87012603
Susceptibility to viruses: herpes simplex * vesic. stomatitis (Indiana)
Description: Rat Wistar-Furth pituitary tumour
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Growth hormone, prolactin Applications: Hormone studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Upon thawing, time before the 1st subculture can be performed may be extended (10-15 days).
Karyotype: Hyperdiploid, Modal No 60
Derived from a 7 month female Wistar-Furth rat. Cells produce growth hormone faster than GH1amd also produces prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 82.1, ICLC: ATL96003
- Endocrinology 1968;82:342 - PMID: 4951281
- In Vitro 1970;6:180 - PMID: 5535574
- J Cell Biol 1969;43:432 - PMID: 5389137
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.