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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute myelomonocytic
DSMZ catalog code: ACC 87
Morphology: single round to oval cells in suspension
Description: human acute myelomonocytic leukemia established in 1979 from the peripheral blood of a 66-year-old woman with a Philadelphia chromosome negative myeloproliferative disorder (resembling chronic myeloid leukemia, CML, in blast crisis) after transformation to refractory acute myelomonocytic leukemia (AML) confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujiasaki Cell Center, Okayama, Japan
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% RPMI 1640 + 20% FBS cells are very difficult to culture! maintain at 0.5-0.8 x 106 cells/ml; cells grow very slowly, split carefully at 37C with 5% CO2 maximum density at 1.2 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hyperdiploid; 48(46-48)<2n>XX, +8, +13, -16, +m, t(2;11)(q36;q13), del(6)(q21), t(7;?)(q35;?), del(12)(p13)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13- CD14- CD15+ CD19- CD33+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ICLC: HTL01008
- Leuk Res 1982;6:743-752 - PMID: 6396552
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.