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DSMZ catalog code: ACC 242
Morphology: adherent, large, spindle-like cells growing in monolayers
Description: human glioma established from the biopsy specimens from a 42-year-old woman with glioma (histologically defined as glioblastoma) confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. O.-B. Tysnes, University of Bergen, Haukeland Hospital, Bergen, Norway
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS + 2 mM L-glutamine + 4x MEM non-essential amino acids split confluent culture 1:5 to 1:6 every 4-6 days using trypsin/EDTA at 37 C with 5-10% CO2 cell harvest of about 10 x 106 cells/80 cm 2;
doubling time of about 40-50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human hypotriploid karyotype with 17% polyploidy; 66(61- 67)<3n>XXX, -4, +5, +7, -10, -13, -19, -22, +dm x1-2, der(1)t(1;4)(q11;p11), del(3)(p14), der(10)(t(3;10)(p14;q13), del(17)(p11), del(18)(p11); additional subclonal rearrangements) * cytogenetics 2 (add(2)(q11), add(7)(q11), i(9q)) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin- desmin- endothel- GFAP- neurofilament- vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase not detected)
- Anticancer Res 1988;8:797-803 - PMID: 3178168
- Anticancer Res 1993;13:1325-1330 - PMID: 8239502
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.