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DSMZ catalog code: ACC 224
Morphology: epithelial, adherent cells, growing in up to 3 layers
Description: mouse urinary bladder established from an adult female C3H/E1 mouse; non-tumorigenic urine bladder cell line immortalized in vitro confirmed as mouse with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. W. I. de Boer, Erasmus University, Rotterdam, The Netherlands
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
45% Ham's F10 + 45% Dulbecco's MEM + 10% FBS + 5 ęg/ml bovine insulin + 5 ęg/ml human transferrin + 50 nM hydrocortisone + 5 ng/ml selenite split confluent culture 1:3 to 1:10 every 3-7 days using trypsin/EDTA at 37C with 5% CO2 harvest of about 0.5 x 105 cells/cm 2;
doubling time of about 35-50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5-2.5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (murine near-tetraploid karyotype with 3.6% polyploidy; 80/81(76-83)<4n>; up to 3 (sub)metacentric markers present in most cells arising by centric fusion) * isoenzymes (confirmed as mouse with IEF of AST, NP) * reverse transcriptase (reverse transcriptase negative)
- Int J Cancer 1993;54:1022-1027 - PMID: 7687588
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.