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SPECIES: mouse, Swiss-Webster
ECACC catalog code: 89050906
Description: Mouse Swiss-Webster myoblast
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Horse Serum (HS) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Myotubes are formed at confluence. This is assisted by reducing both sera to 4%.
Isolated from a myogenic line derived from hind limb muscle of foetal Swiss-Webster mouse. Non-tumorigenic and highly sensitive to acetylcholine. Confluent cultures form multinucleated myotubes.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1456
- Science 1977;196:995 - PMID: 193191
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.