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ICLC catalog code: HTL96003
Continuous culture, grown as monolayer
polygonal flat to spindle-like morphology
SPECIES: human (male, 42 years old) Karyology: complex chromosomal changes; p53 mutation
TUMOR: carcinoma, follicular
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: The cells were used for studies on the function of human thyroid, development of thyroid cancer, expression of 5-deiodinase type I
Available in the following LABORATORY:
- IRCCS AOU San Martino -IST Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + Ham's F12 (1:1) + 5% FBS + 2 mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-4 x 104 cells/cm2. 37C, 5% CO2. Passage: 53, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with MD, NP)
Availability in cell line catalogues: ECACC: 94060902
- Cancer Res 1990;118:48 - PMID: 2152773
- Front Horm Res 1989;18:56
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.