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ECACC catalog code: 90102525
Description: Bull frog tongue
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Distilled H2O + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.02% EDTA at 10C; 5% CO2; 25C. Capable of growth down to 4C.
Karyotype: Modal No 43
Derived from tongue of normal female bull frog. Incubation is optimal at 25C but growth at a slower rate can continue even at 4C where cells may be stored for prolonged periods.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 41
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.