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SPECIES: mouse, C3H
ECACC catalog code: 87111904
Description: Mouse C3H mammary carcinoma
Viruses: See Properties
Depositor: Dr T Ohno, Riken Gene Bank, Tsukuba, JAPAN
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies, chromosome condensation studies.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 1-3x100,000 cells/ml; 5% CO2; 33C.
A temperature sensitive mutant isolated from the mouse mammary cell carcinoma cell line, FM3A (ECACC No. 87100804). Cells will Not double at 39C (Non-permissive temperature) and will grow at 33C (permissive temperature). Electron Microscope findings have shown that chromatin abnormally condenses into fragmented and compact forms in 80% of the cells at 39C for 16 hours. This abnormal condensation does Not occur at 33C or in the FM3A (ECACC No. 87100804) cell line at either temperature.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Exp Cell Res 1980;126:407-416 - PMID: 6153988
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.