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TISSUE/ORGAN: kidney, embryonic
DSMZ catalog code: ACC 153
Morphology: fibroblast-like cells growing as monolayers
Susceptibility to viruses: bovine leukemia
Description: ovine embryonal kidney cells established from the embryonal kidney cells of an ovine in 1976; cells are susceptible to infection with BLV (bovine leukemia virus) leading to the formation of syncythia confirmed as ovine with IEF of AST, LDH, NP
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. R. Riebe, Friedrich-Loeffler-Institute, Insel Riems, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% MEM (with Earle's salts) + 10% FBS + 850 mg/L NaHCO 3 + 120 mg/L sodium pyruvate + 1x non-essential amino acids split confluent culture 1:2 to 1:6 every 2 days using trypsin/EDTA at 37C with 5% CO2;
frozen with 82.5% medium, 10% FBS, 7.5% DMSO at about 0.2-0.4 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.