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ECACC catalog code: 90111910
Susceptibility to viruses: many types of viruses * poliovirus 1
Description: Human amnion, HeLa characteristics
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus, tumourigenicity and chromosome studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Karyotype: Modal No 71
Derived from Normal amniotic tissue. Have been used and studied extensively including virus susceptibility, tumorigenicity and morphology. Contains HeLa marker chromosomes and exhibits type A G6PD This cell line was found to have a HeLa profile by DNA fingerprinting at ECACC. The cells are capable of growth in a variety of media supplemented with sera from several species.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 62, IZSBS: BS CL46
- Arch Ges Virus Forsch 1960;9:559
- Cancer Res 1958;18:692
- J Nat Cancer Inst 1959;23:893
- Proc Soc Exp Biol Med 1957;94:532
- Proc Soc Exp Biol Med 1965;119:223
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.