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TUMOR: carcinoma, undifferentiated
DSMZ catalog code: ACC 219
Morphology: epithelial-like adherent cells growing as monolayer
Description: human thyroid carcinoma established from the primary tumor of a 78-year-old woman with thyroid carcinoma (undifferentiated carcinoma, histologically a largely papillary adenocarcinoma with some spindle, polygonal and giant cells) confirmed as human with IEF of AST, LDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. M. Akiyama, Radiation Effects Research Foundation, Hiroshima, Japan
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture 1:5 every 2-3 days or 1:10 every 4-5 days using trypsin/EDTA at 37C with 5% CO2 cell harvest of about 20 x 10 6 cells/175 cm 2;
doubling time of about 30-40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human hypotriploid karyotype with 16% polyploidy; 65(57- 68)<3n>XX,-X, -2, +3, -7, -10, +11, -13, -16, -18, -19, +2mar, del(1)(q13), del(3)(q22), ?del(4)(q21), i(8q)) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin+) * reverse transcriptase (reverse transcriptase not detected)
Availability in cell line catalogues: ECACC: 94090184
- Cancer Res 1992;52:1369 - PMID: 1737400
- Int J Oncol 1994;4:583-586
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.