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ICLC catalog code: HTL96026
Continuous culture, grown as monolayer
large spindle-shaped cells morphology
SPECIES: human (female, 67 years old)
TUMOR: carcinoma, undifferentiated
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:4 once a week using trypsin/EDTA. Doubling time is about 43 hours. Seed at 2-4 x 104 cells/cm2; cell harvest is about 2 x 106 cells/150 cm2 flask. 37C, 5% CO2., mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with AST, MD)
Availability in cell line catalogues: ECACC: 94090183, DSMZ: ACC 133
- Biochem Biophys Res Commun 1994;198:948-954 - PMID: 8117301
- Cancer Res 1992;52:1369 - PMID: 1737400
- Int J Oncol 1994;4:583-586
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.