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TUMOR: erythroleukemia, lymphoblast
ECACC catalog code: 87030901
Description: Mouse erythroleukaemic lymphoblast
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Haematopoiesis studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F10 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Karyotype: 2n = 22
This cell line is a clone isolated from the parent line 745 TG-12. The cells are resistant to oubain and thioguanine and deficient in HGPRT. Hemoglobulin synthesis can be induced with 1.5% DMSO or 1mM oubain.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Cell 1977;12:653 - PMID: 922887
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.