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SPECIES: mouse / mouse
DSMZ catalog code: ACC 89
Morphology: single cells in suspension
Description: mouse hybridoma (anti-EBV) established by fusion of the P3X62AG8.653 mouse myeloma cell line with spleen cells of a BALB/c mouse immunized with Epstein-Barr virus (EBV) particles; cells produce a monoclonal antibody (IgG 1 ) that reacts with a viral envelope glycoprotein (350/220 kD) and that neutralizes EBV confirmed as mouse with IEF of AST, MDH, PEP B
Viruses: ELISA: reverse transcriptase positive
Depositor: obtained from ATCC (HB 168), Rockville, Maryland, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.1-1.0 x 106 cells/ml; split every 2-3 days at 1:3 to 1:5 at 37C with 5% CO2 1.0-1.5 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: isoenzymes (confirmed as mouse with IEF of AST, MDH, PEP B) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: HB 168
- Proc Natl Acad Sci U S A 1980;77:2979-2983 - PMID: 6248876
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.