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SPECIES: mouse, (C57BL/6xDBA/2)F1
TUMOR: leukemia, pre-B cell
ECACC catalog code: 91092002
Transformed by: Chemical Agent Back To Chemical Agents' WebPage
Description: Mouse pre-B lymphocyte
Viruses: See Properties
Depositor: Dr P Kincade, Oklahoma Medical Research Foundation, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Cytoplasmic Immunoglobulin
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Fetal Bovine Serum (FBS). Maintain cultures between 2-5x100,000 cells/ml; 5% CO2; 37C.
Derived from a methyl nitrosourea-induced tumour in a (C57BL/6XDBA/2) F1 mouse. This line is positive for cytoplasmic Ig and negative for surface membrane Ig and TdT.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: TIB 158
- J Immunol 1978;121:641 - PMID: 308074
- Leuk Res 1979;3:23 - PMID: 316844
- Nature 1981;292:631 - PMID: 6789213
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.