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SPECIES: mouse, 129
TUMOR: carcinoma, embryonal
DSMZ catalog code: ACC 112
Morphology: adherent cells with undifferentiated morphology growing in clumps
Description: mouse embryonal carcinoma initiated from a testicular teratocarcinoma of a strain 129 mouse confirmed as mouse with IEF of AST, MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: obtained from ATCC (CRL 1720), Rockville, Maryland, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM (4.5 g/L glucose) + 10% FBS presoak flasks with 0.1% gelatin at 4C for 2 hours, wash 3x with PBS; split confluent culture 1:10 to 1:20 every 2-4 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm 2 at 37C with 5% CO2 about 70 x 106 cells/80 cm 2;
doubling time of ca. 24 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near-diploid karyotype with 6% tetraploidy; 38(37-40)<2n>; 1-2 centric fusion markers present) * isoenzymes (confirmed as mouse with IEF of MDH, NP, PEP B) * reverse transcriptase (reverse transcriptase negative (RDDP assay))
Availability in cell line catalogues: ATCC: CRL 1720, ECACC: 85061803, ICLC: ATL99006
- Cell 1978;15:393-403 - PMID: 214238
- Cell 1980;21:347 - PMID: 6250719
- Exp Cell Res 1980;126:407-416 - PMID: 6153988
- Proc Natl Acad Sci U S A 1973;70:3899 - PMID: 4521215
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.