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SPECIES: mouse, (C57BL/10xC57BL/10.BR)
TISSUE/ORGAN: lymphocyte, T
DSMZ catalog code: ACC 52
Morphology: single or clustered cells in suspension
Description: mouse T cells established from a bovine insulin-specific CD4+ T cell line (C57Bl/10 x C57Bl/10.BR H-2 b x H-2 k ); cell line can be used as assay for IL-4
Depositor: Dr. E. Schmitt, Institute of Immunology, Mainz, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
CG medium + 50 U/ml mouse IL-4 (strictly without FBS) maintain at 0.2-0.4 x 10 6 cells/ml; split 1:2; upon thawing of frozen cells, cells must be washed thoroughly as already tiny amounts of FBS willl lead to adherence at 37C with 5% CO2 saturation density at about 0.5 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture assays;
- Eur J Immunol 1990;20:1709-1715 - PMID: 2120063
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.