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SPECIES: mouse / mouse
TUMOR: hybridoma, B cell
DSMZ catalog code: ACC 23
Morphology: round cells, growing single or clustered in suspension
Description: mouse B cell hybridoma murine myeloma cells fused with C57Bl/6 spleen cells; indicator cell line for IL-6 confirmed as mouse with IEF of MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. E. Schmitt, Institute of Immunology, University of Mainz, Mainz, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 5% FBS + 5% ConA spleen cell supernatant (or 25 U/ml IL-6; or 10% vol conditioned medium of cell line 5637 (DSM ACC 35) + 2.5 x 10-5 M mercaptoethanol + 2 mM L-glutamine maintain at 0.1-0.3 x 106 cells/ml, optimal split ratio (daily) of 1:3, maximal split ratio of 1:5 at 37C with 7.5% CO2 maximum density of 0.5-1.0 x 106 cells/ml;
doubling time of ca. 24-25 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 105 cells/ampoule;
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: immunological analysis (convenient bioassay for IL-6) * isoenzymes (confirmed as mouse with IEF of MDH, NP, PEP B)
Availability in cell line catalogues: ATCC: CRL 1851
- Proc Natl Acad Sci U S A 1986;83:9679-9683 - PMID: 2948184
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.