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TISSUE/ORGAN: bone marrow
TUMOR: leukemia, pre-B cell
DSMZ catalog code: ACC 42
Morphology: round, single cells and clusters of cells in suspension
Endogenous viruses: contains Epstein-Barr
Description: human B cell precursor leukemia established from the bone marrow of a 12-year-old boy with acute lymphoblastic leukemia (cALL) at relapse in 1979; cells express bcl-2, bcl-3 and c-myc mRNA; recently termed 'EU-3' in the literature confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. M. Cooper, University of Alabama, Birmingham, Alabama, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-1.0 x 106 cells/ml at 37C with 5% CO2 maximal density at about 2-3 x 106 cells/ml;
doubling time of about 30-40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 ( 46(45-48)<2n>XY, t(1;19)(q23;p13), del(6)(q21) human near diploid karyotype) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10+ CD13- CD19+ CD37- HLA-DR+ cyIgM+ sIgM+ cy/sIgG- cy_-cy_-) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (not detected (RDDP assay))
- Blood 1982;60:1305-1308 - PMID: 6982733
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.