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SPECIES: human, Caucasian
TISSUE/ORGAN: bone marrow
TUMOR: leukemia, chronic myeloid
DSMZ catalog code: ACC 134
Morphology: single, round to oval cells in suspension
Description: human chronic myeloid leukemia in blast crisis established from the bone marrow of a 5-year-old Caucasian girl with Philadelphia chromosome-positive CML (chronic myeloid leukemia) in second relapse in 1980 (after bone marrow transplantation day +47); sister cell line of EM-2 (DSM ACC 135) confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. A. Keating, Toronto General Hospital, Ontario, Canada
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-1.5 x 106 cells/ml; subculture at a 1:2 to 1:3 dilution every 48 hours; seed out at ca. 0.5 x 106 cells/ml at 37C with 5% CO2 grows up to about 2 x 106 cells/ml;
doubling time of ca. 40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypertriploid; 73(69-77)<3n>X, -X, -X, t(9;22)(q34;q11), i(17q)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile, but similar to EM-2 (DSM ACC 135)) * immunological analysis (CD3- CD13+ CD14- CD15+ CD19- CD33+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (reverse transcriptase negative)
- Cancer Genet Cytogenet 1987;25:271-284 - PMID: 3030532
- Clin Haematol 1987;1:1021-1029
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.