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SPECIES: mouse, C3H/HeNSIc
DSMZ catalog code: ACC 44
Morphology: large, round, single cells in suspension
TRANSFORMED BY: Radiant Agent Back To Radiant Agents' WebPage
Description: mouse erythroleukemia established from an X-irradiated C3H/HeNSIc mouse; cells were described to be stroma-independent, responsive to erythropoietin and to produce hemoglobin (augmented by IL-3) confirmed as mouse with IEF of MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. K. J. Mori, Niigata University, Niigata, Japan
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% alpha-MEM + 10% FBS maintain at 0.1-0.5 x 106 cells/ml; split 1:3 at 37C with 5% CO2 saturation density of about 0.5-1.0 x 106 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: isoenzymes (confirmed as mouse with IEF of MDH, NP, PEP B)
- Leuk Res 1988;12:471-478 - PMID: 2457136
- Leuk Res 1990;14:287-291 - PMID: 2319808
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.