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SPECIES: mouse / human
ECACC catalog code: 86072804
Description: Human x mouse hybrid
Viruses: See Properties
Depositor: Dr P Scambler, Institute of Child Health, London, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: pJ3.11, p79a, CFTR DNA markers Applications: Somatic cell hybrid mapping
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 20% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 37C; 5% CO2.
This cell line may contain sub-chromosomal fragment of human chromosome 17 bearing markers thought at the time to flank the cytotic fibrosis gene. The human DNA was donated from the human osteosarcoma cell line MNNG-HOS.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Nucleic Acids Res 1986;14:7159-7174 - PMID: 3763403
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.