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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, chronic, B cell
DSMZ catalog code: ACC 67
Morphology: lymphoblastoid, round to polygonal, single or clustered cells in suspension
TRANSFORMED BY: Epstein-Barr
Description: human chronic B cell leukemia established from the peripheral blood of a 69-year-old woman with B-CLL (chronic lymphocytic leukemia) prior treatment by EBV-transformation in 1988; cells were described to be EBV+, CD5+ and to express mRNA of bcl-2, bcl-3 and c-myc confirmed as human with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. K. Guy, Western General Hospital, Edinburgh, UK
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.1-0.5 x 106 cells/ml; split at ca. 1:2 every 2-3 days; cells may grow slowly initially; use initially 24-well plate culture dishes and 20% FBS; seed out at ca. 0.5 x 106 cells/ml at 37C with 5% CO2 maximal density at about 1.0 x 106 cells/ml;
doubling time of ca. 50-70 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypodiploid karyotype with 1.6% polyploidy; 45(42-46)<2n>X, -X; no structural rearrangements detected) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13- CD19+ CD37+ HLA-DR+ smIgG+) * isoenzymes (confirmed as human with IEF of MDM, NP)
- Leuk Res 1990;14:381-387 - PMID: 2159091
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.