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DSMZ catalog code: ACC 191
Morphology: adherent, epithelial-like cells growing as monolayers
Description: human ovary adenocarcinoma established from the solid omental metastasis of a mucinous papillary adenocarcinoma of the ovary of a 36-year-old woman in 1979; initially diploid, later tetraploid; growth in nude mice; cells were received at passage 48, frozen at DSMZ at passage 53-57 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Prof. Dr. F. Hoelzel, Women's University Hospital Eppendorf, Hamburg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% RPMI 1640 + 20% FBS + 2 mM L-glutamine + 1x MEM non-essential amino acids + 1 mM sodium pyruvate split confluent culture 1:2 to 1:5 every 2-5 days using trypsin/EDTA (incubate for 10-20 minutes with trypsin/EDTA); seed out at ca. 1.5 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of about 0.3-0.6 x 10 5 cells/cm 2;
doubling time of about 50-80 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5-2.5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (human flat-moded hypotetraploid karyotype with 17% polyploidy; 78-91<4n>XXXX, -4, -10, -13, -13, -19, +mar; i(9q), 16qh+x2; extensive subclonal rearrangements, e.g. der(5)t(5;7)(q31.2;q11)) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+ (100%) desmin- endothel- GFAP- neurofilament- vimentin+ (50%)) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase not detected)
- Cancer Genet Cytogenet 1987;28:201-212 - PMID: 3476184
- J Natl Cancer Inst 1983;70:839-845 - PMID: 6405069
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.