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SPECIES: human, Caucasian
DSMZ catalog code: ACC 235
Morphology: epitheloid adherent cells growing in monolayers
Description: human ovary cystadenocarcinoma established from the ascitic fluid of a 56-year-old Caucasian woman with dedifferentiated serous cystadenocarcinoma of the ovary in 1979; cells were received at passage 152, frozen at DSMZ at passage 164 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Prof. Dr. F. Hoelzel, Women's University Hospital Eppendorf, Hamburg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% RPMI 1640 + 20% FBS + 2 mM L-glutamine + 1x MEM non-essential amino acids + 1 mM sodium pyruvate split confluent culture once a week using trypsin/EDTA; seed out about 1-2 x 106 cells/175 cm 2 , after thawing cells might at first grow very slowly at 37C with 5% CO2 cell harvest of about 6 x 106 cells/175 cm 2;
doubling time of about 43 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 0.5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (apparently human karyotype) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+ (100%) desmin- endothel- GFAP- neurofilament- vimentin+ (100%)) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
- J Natl Cancer Inst 1983;70:839-845 - PMID: 6405069
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.