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SPECIES: human, Caucasian
DSMZ catalog code: ACC 230
Morphology: epitheloid cells growing in monolayers
Description: human endometrium carcinoma established from the ascitic fluid of a 69-year-old Caucasian woman with endometrial carcinoma in relapse in 1985; cells were received at passage 91, frozen at DSMZ at passage 99 confirmed as human with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Prof. Dr. F. Hoelzel, Women's University Hospital Eppendorf, Hamburg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture at 1:4 about once a week using trypsin/EDTA; cells grow very slowly; seed out about 1 x 106 /175 cm 2 at 37C with 5% CO2 cell harvest of about 4-5 x 106 cells/175 cm 2;
doubling time of about 92 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 0.8 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (human flat-moded hypertriploid karyotype with 1-2 supernumerary dm in most cells and 23% polyploidy; 80(72-81)<3n>XX,-X, +1, +2, -4, -5, +6, +8, +10, -13, +17, +19, +20, +21, +6 mar; der(1)t(1;?)((p11;?)x2, del(2)(q24)x2, der(2)t 2;3)) * cytogenetics 2 ((q11;p11), del(12)(p13), der(16)t(16;?)(q21/23;?), der(17)t(7;17)(q11;p11), add(22)(p11)x2) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+, desmin-, endothel-, GFAP (+), neurofilament-, vimentin+) * isoenzymes (confirmed as human with IEF of AST, NP) * reverse transcriptase (reverse transcriptase not detected)
- 15th International Cancer Congress Hamburg Abstract 1990;2.070.09
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.