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SPECIES: human, Black
TUMOR: lymphoma, Burkitt
DSMZ catalog code: ACC 80
Morphology: round, single or aggregated cells in suspension
Description: human Burkitt's lymphoma established from the maxilla tumor lymph node of a 9-year-old black girl with Burkitt's lymphoma in 1964; cells were described to be EBV-positive confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS cells grow very fast; maintain at 0.5-1.0 x 106 cells/ml; split 1:2 to 1:3 at 37C with 5% CO2 maximal density at 1.0-2.0 x 10 6 cells/ml;
frozen with 70% medium, 20% FBS, 10% DMSO at 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hyperdiploid karyotype with 15% polyploidy; 47(44-48)<2n>XX/XXX, +7, t(8;14)(q24;q32), der(11)t(11;?)(q22;?), der(19)t(19;?)(q13;?); sideline carries additional ch 2) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10+ CD13- CD19+ CD37+ HLA-DR+ cyIgG- cyIgM-) * isoenzymes (confirmed as human with IEF of AST, MDH, NP)
Availability in cell line catalogues: ATCC: HTB 60
- J Natl Cancer Inst 1965;34:231-240
- J Natl Cancer Inst 1965;34:241-253
- Lancet 1964;1:252-253
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.