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SPECIES: human, Caucasian
PATHOLOGY: infectious mononucleosis (OMIM
ECACC catalog code: 90121307
TRANSFORMED BY: Epstein-Barr
Description: Human Caucasian infectious mononucleosis lymphocytes
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Transformation and virus studies: EBV
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 20% Fetal Bovine Serum (FBS). Maintain cultures between 4-5x100,000 cells/ml; 5% CO2; 37C. Subculture every 8 days by centrifugation. Max of 1-2 x 1,000,000 viable cells/ml obtainable.
Karyotype: 2n = 46
Established by seeding peripheral blood lymphocytes from 20-yr old female, 2 months after onset of infectious mononucleosis. EBV transformed B-lymphoblastoid line. The cells should be handled under laboratory containment level 2. Cells are particularly resistant to poliomyelitis and vesicular stomatitis, viruses similar to other cell lines carrying EB virus.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 104
- J Am Med Assoc 1968;203:205
- Proc Natl Acad Sci U S A 1968;59:94 - PMID: 5242134
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.