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ECACC catalog code: 88032803
Susceptibility to viruses: coronavirus * equine infectious anaemia virus * herpes simplex * reovirus 3 * vesicular stomatitis (Ogden strain)
Description: Horse dermis
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Finite lifespan of approx. 40 passages.
Karyotype: 2n = 64
Derived from a biopsy of a 4 year old mare. These cells have a finite lifespan of a total of 40 passages. Susceptible to herpes simplex, reovirus type III, vaccinia and vesicular stomatitis viruses. Not susceptible to human adenovirus type V, coxsackie A9 and B5 and polio type II.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 57, IZSBS: BS CL 40, ICLC: AL97004
- Proc Soc Exp Biol Med 1958;98:574
- Vet Rec 1983;262
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.