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SPECIES: rat, WAG/RIJ
TISSUE/ORGAN: thyroid, medullary
ECACC catalog code: 87042206
Description: Rat medullary thyroid carcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Neurotensin and calcitonin Applications: Hormone studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Derived from non-clonal line rMTC 6-23 originally established from a transplantable WAG/RIJ rat medullary thyroid carcinoma. The cell line synthesises and secretes both neurotensin and calcitonin. Its clonal derivation demonstrates that a population derived from a single type of C-cell has the capability of producing both peptides. Secretion of these peptides has been shown to be stimulated by calcium, KC1, glucagon and catecholamines.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1607, IZSBS: TCL 130
- Endocrinology 1980;107:509 - PMID: 6248326
- Proc Natl Acad Sci U S A 1980;77:3741 - PMID: 6932046
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.