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Monoclonal continuous culture, grown as suspension
SPECIES: mouse, DBA/2
Original line: DS19
Transformed by: mitomycin C mutagenesis Back To Mutations WebPage
tumorigenic in nude mice
Endogenous viruses: contains Friend leukemia virus
Cell line available under some conditions.
PROPERTIES: differentiation; genetics
Available in the following LABORATORY:
- Dip. Genetica-Biologia Generale e Molecolare (NAUCC, Napoli)
DMEM + 7.5% FBS + Antibiotics + 2mM L-Glutamine; Freezing medium: Culture medium + 20% FBS + 15% Glycerol; 37C, 10% CO2, split 1-10, mycoplasma negative
Undifferentiated cells and proerythroblasts morphology. Erythroid differentiation blocked by mitomycin C.
Validated by: biochemical assays * cytochemical assays
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.