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TUMOR: lymphoma, B cell
DSMZ catalog code: ACC 47
Morphology: cells grow as single cells or in clumps in suspension
Description: human B cell lymphoma established from the pleural effusion of a 60-year-old man with refractory immunoblastic B cell lymphoma progressed from follicular centroblastic/centrocytic lymphoma in 1990; cells were described to carry the 14;18 chromosomal translocation (bcl-2 rearranged, mbr) and to express bcl-2 mRNA confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. C. Kluin-Nelemans, University Medical Center, Leiden, The Netherlands
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.3-0.7 x 106 cells/ml; split 1:2 every second day; seed out at ca. 0.2-0.4 x 106 cells/ml at 37C with 5% CO2 maximal density of about 0.7-1.0 x 106 cells/ml;
doubling time of ca. 40 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hyperdiploid karyotype; 47(43-48)<2n>XY, +7, der(8)t(8;18)(q24;q21), der(14)t(8;14)(q24;q32), der(18)t(14;18)(q32;q21); earliest of a recent series of cell lines with cohabiting 8;14 (Burkitt or diffuse large cell lymphoma) and 1 ;18) * cytogenetics 2 ((follicular lymphoma) translocations) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10+ CD13- CD19+ CD37+ HLA-DR+ smIg/cyIgG+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (negative for reverse transcriptase, HTLV-I/II, HIV-1)
- Leukemia 1991;5:221-224 - PMID: 1849602
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.