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ECACC catalog code: 90102523
TRANSFORMED BY: murine sarcoma virus
Description: Canine kidney, Moloney Sarcoma Virus infected
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: Murine MSV, RNA and xenotropic human tumour viruses
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
McCoy's 5a + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/0.02% EDTA; 5% CO2; 37C.
Karyotype: 2n = 78
Derived by the infection of a sub-clone of MDCK (ECACC No. 84121903) with murine MSV. The cells are used for studies of the interaction between RNA tumour viruses and mammalian cells including the isolation of xenotropic human tumour virus.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 34.1
- Virology 1975;67:344 - PMID: 52940
- Virology 1976;70:313 - PMID: 57666
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.