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TUMOR: carcinoma, small cell
ECACC catalog code: 95062824
Morphology: Irregularly shaped aggregates
Description: Human lung small cell carcinoma
Viruses: See Properties
Depositor: Prof OS Pettengill and Prof. GD Sorenson, Dartmouth Medical School, Lebanon, NH, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Human lung tumour model, carcinogenesis studies.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS) (Heat Inactivated). Split confluent cultures 1:2 to 1:4 i.e. seeding at 1-2x100,000 cells/cm2; 5% CO2; 37C.
DMS79 has been established from pleural fluid of a patient with small cell carcinoma of the lung. The patient was treated with cytoxan, vincristine, methotrexate and radiation therapy. The cultures show a tendency for the modal chromosome number to decline. The production of adrenocorticotropin (ACTH), bombesin, calcitonin, beta-endorphin, 17-beta-estradiol, lipotropin, ocytocin-neurophysin, parathyroid hormone and somatostatin-like immuno-reactivity has been reported. The cells have also been reported to have the following cell surface antigens: Leu-7, Class I HLA, Class II HLA, Mg23 defined by the AML-2-23 monoclonal antibody and the AML-1-99 defined antigen. The cells have a receptor for epidermal growth factor (EGF) and produce tumours in nude athymic mice.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 2049
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.