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TUMOR: carcinoma, small cell
ECACC catalog code: 95062830
Description: Human lung, small cell carcinoma
Viruses: See Properties
Depositor: Prof OS Pettengill and Dr G D Sorenson, Dartmouth Medical School, Lebanon, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Human lung tumour model
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Waymouth's MB 752/1 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS) (Heat Inactivated). Split confluent cultures 1:4 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
DMS 273 was initiated in 1978 from the pleural fluid specimen from a 50 year old female patient with small cell carcinoma of the lung. The patient was in relapse after chemotherapy and radiotherapy. DMS 273 cells exhibit intercellular processes and express retinoblastoma mRNA and protein. The cell line is tumorigenic in nude mice.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Cancer Res 1990;50:5312 - PMID: 2167152
- Exp Cell Biol 1980;48:279
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.