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ECACC catalog code: 90062901
Description: Rat colonic carcinoma
Viruses: See Properties
Depositor: Dr Martin, Immunology Laboratory, Faculty of Medicine, INSERM, Dijon, FRANCE
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Metastasis studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + F10 (1:1) + 2mM Glutamine + 10% Fetal Bovine Serum (FBS) or 2% Rat Serum. Split confluent cultures 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
This cell line has properties essential for study of metastasis of colon carcinomas; a reproducible pattern of metastatic behaviour and ability to be propagated both in vitro and in vivo to facilitate identification of biochemical correlates of metastasis.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Int J Cancer 1983;32:623 - PMID: 6358055
- Int J Cancer 1987;39:248 - PMID: 3804494
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.