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TUMOR: lymphoma, Burkitt
DSMZ catalog code: ACC 83
Morphology: round to polygonal, single or clustered cells in suspension
Description: human Burkitt's lymphoma established from the pleural effusion of a 10-year-old boy with Burkitt's lymphoma (refractory, terminal) in 1975 confirmed as human with IEF of AST, MDH, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. Ben-Bassat, Hadassah Medical Organization, Jerusalem, Israel
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.1-0.5 x 106 cells/ml; split 1:5 to 1:10 every 24-48 hours; seed out at ca. 0.5 x 106 /ml at 37C with 5% CO2 saturation density at 0.75-1.0 x 106 cells/ml;
doubling time of ca. 40-50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at 5 x 106 cells/ampoule;
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (near diploid with 6% polyploidy; 46<2n>XY, t(8;14)(q24;q32)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD10+ CD13+ CD19+ CD37+ smIgM+ smIgG-) * isoenzymes (confirmed as human with IEF of AST, MDH, PEP B) * reverse transcriptase (reverse transcriptase negative)
- Int J Cancer 1977;19:27-33 - PMID: 188769
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.