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DSMZ catalog code: ACC 35
Morphology: epithelial-like adherent cells
Description: human urinary bladder carcinoma established from the primary bladder carcinoma of a 68-year-old man in 1974; cells were described to produce several growth factors (e.g. SCF, IL-1, IL-6, G-CSF, GM-CSF, etc.) confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Prof. K. Welte, Medical School of Hannover, Hannover, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent cultures 1:4 to 1:5 using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm 2 at 37C with 5% CO2 about 8-10 x 10 6 cells/80 cm 2;
doubling time of ca. 24 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypotriploid with 17% polyploidy; 62(47-64)<3n>XX, -Y, -4, -5, -10, -13,-13, -15, -18, -19, -21, -21, +4mar, der(1)t(1;?)(p21;?), der(3)t(3;?)(p22;?), del(6)(q23), der(9)t(9;?)(p12;?), der (11)t(5;11)(q14;p12), ?der(12)t(12;) * cytogenetics 2 (i(13q), i(14q), der(17)t(17;?)(p11/12;?), del(22)(q13); carries large submetacentric marker with q arm resembling tandem dup(7); 9p12 is a recurrent breakpoint in bladder carcinoma.) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: HTB 9, ECACC: 90110524, ICLC: HTL01017
- Proc Natl Acad Sci U S A 1985;82:1526-1530 - PMID: 3871951
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.