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SPECIES: monkey, rhesus
TISSUE/ORGAN: lung, embryonic
ECACC catalog code: 89101303
Susceptibility to viruses: Coxsackie A9 * parainfluenza 3 * poliovirus 1 * poliovirus 2 * poliovirus 3 * rubella
Description: Monkey Rhesus lung
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: This material is cited in a US and/or other Patent and may not be used to infringe parent claims. US Patent No. 4,040,905
Properties: Applications: Virus studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C. Finite lifespan. Cell line has been shown to undergo 51 passages or 74 population doublings.
Karyotype: 2n = 42, diploid no structural abnormalities observed
A diploid cell line derived from normal male Rhesus monkey foetus. Susceptible to poliovirus types 1-3, Coxsackie A9, parainfluenza 3, rubella and is non-tumourigenic in animals.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 160
- In Vitro 1973;8:323 - PMID: 4348695
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.