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SPECIES: monkey, African green
TISSUE/ORGAN: lung, embryonic
ECACC catalog code: 90112815
Susceptibility to viruses: Coxsackie A9 * herpes simplex * mumps * parainfluenza 3 * poliovirus * rhinovirus * rubella * vaccinia
Description: Monkey African Green lung
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: This material is cited in a US and/or other Patent and may not be used to infringe parent claims. US Patent No. 4,040,905
Properties: Applications: Virus studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C. Finite lifespan, up to 12 population doublings are expected.
Karyotype: 2n = 60, diploid
Derived from 141 day old normal male African Green Monkey foetus. Free of adventitious agents and are non-tumourigenic. Susceptible HSV, vaccinia, rhinovirus, mumps, rubella, parainfluenza 3, Coxsackie A9 and poliovirus types 1-3.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 162
- In Vitro 1973;8:333 - PMID: 4633070
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.